Optimizing the transient transfection process of HEK-293 suspension cells for protein production by nucleotide ratio monitoring

Large scale, transient gene expression (TGE) is highly dependent of the physiological status of a cell line. Therefore, intracellular nucleotide pools and ratios were used for identifying and monitoring the optimal status of a suspension cell line used for TGE. The transfection efficiency upon polye...

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Bibliographic Details
Main Authors: de los Milagros Bassani Molinas, Maria, Beer, Christiane, Hesse, Friedemann, Wirth, Manfred, Wagner, Roland
Format: Artigo
Language:Inglês
Published: Springer Netherlands 2013
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Original Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3973801/
https://ncbi.nlm.nih.gov/pubmed/23775287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9601-3
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