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Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is espec...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1985
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397316/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856838/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.6.1585 |
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