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Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is espec...

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Wood, W I, Gitschier, J, Lasky, L A, Lawn, R M
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1985
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397316/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856838/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.6.1585
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