Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.

Two simple alternatives to the synthesis of mixed oligodeoxyribonucleotide probes are described: mixed deblocking of triazolodeoxynucleosides for the A/G and C/T degeneracies or incorporation of 2-amino-2'-deoxyadenosines into a determined sequence for a higher stability of the hybridization duplexe...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Huynh-Dinh, T, Duchange, N, Zakin, M M, Lemarchand, A, Igolen, J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390846/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3865174/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.22.7510
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