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Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.
Two simple alternatives to the synthesis of mixed oligodeoxyribonucleotide probes are described: mixed deblocking of triazolodeoxynucleosides for the A/G and C/T degeneracies or incorporation of 2-amino-2'-deoxyadenosines into a determined sequence for a higher stability of the hybridization du...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1985
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC390846/ https://ncbi.nlm.nih.gov/pubmed/3865174 |
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