A fidelity assay using "dideoxy" DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
DNA replication fidelity has been assayed by using a modified DNA sequencing reaction. In one experimental approach, dideoxycytidine 5'-triphosphate (ddCTP) was used as a chain terminator during replication of M13 phage DNA by the large fragment of DNA polymerase I. The deoxyribonucleotide analogue...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1985
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397248/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3856263/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.5.1301 |
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