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Kinetic measurement of 2-aminopurine X cytosine and 2-aminopurine X thymine base pairs as a test of DNA polymerase fidelity mechanisms.

Enzyme kinetic measurements are presented showing that Km rather than maximum velocity (Vmax) discrimination governs the frequency of forming 2-aminopurine X cytosine base mispairs by DNA polymerase alpha. An in vitro system is used in which incorporation of dTMP or dCMP occurs opposite a template 2...

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Hlavní autoři: Watanabe, S M, Goodman, M F
Médium: Artigo
Jazyk:Inglês
Vydáno: 1982
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC347139/
https://ncbi.nlm.nih.gov/pubmed/6959128
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