Genome engineering using the CRISPR-Cas9 system

Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in eukaryotic ce...

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Main Authors: Ran, F Ann, Hsu, Patrick D, Wright, Jason, Agarwala, Vineeta, Scott, David A, Zhang, Feng
Format: Artigo
Jezik:Inglês
Izdano: 2013
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3969860/
https://ncbi.nlm.nih.gov/pubmed/24157548
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.143
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