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Genome engineering using the CRISPR-Cas9 system
Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate efficient genome engineering in eukaryotic ce...
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| Autori principali: | , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3969860/ https://ncbi.nlm.nih.gov/pubmed/24157548 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.143 |
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