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Structural elements responsible for conversion of streptavidin to a pseudoenzyme

Avidin enhances the hydrolysis of biotinyl p-nitrophenyl ester (BNP) under mild alkaline conditions, whereas streptavidin prevents hydrolysis of BNP up to pH 12. Recently, we imposed hydrolytic activity on streptavidin by rational mutagenesis, based on the molecular elements responsible for the hydr...

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Publicado no:Proc Natl Acad Sci U S A
Principais autores: Eisenberg-Domovich, Yael, Pazy, Yael, Nir, Orit, Raboy, Bilha, Bayer, Edward A., Wilchek, Meir, Livnah, Oded
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC395898/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15079055/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0308541101
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