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Structural elements responsible for conversion of streptavidin to a pseudoenzyme
Avidin enhances the hydrolysis of biotinyl p-nitrophenyl ester (BNP) under mild alkaline conditions, whereas streptavidin prevents hydrolysis of BNP up to pH 12. Recently, we imposed hydrolytic activity on streptavidin by rational mutagenesis, based on the molecular elements responsible for the hydr...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC395898/ https://ncbi.nlm.nih.gov/pubmed/15079055 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0308541101 |
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