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Structural elements responsible for conversion of streptavidin to a pseudoenzyme

Avidin enhances the hydrolysis of biotinyl p-nitrophenyl ester (BNP) under mild alkaline conditions, whereas streptavidin prevents hydrolysis of BNP up to pH 12. Recently, we imposed hydrolytic activity on streptavidin by rational mutagenesis, based on the molecular elements responsible for the hydr...

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書誌詳細
出版年:Proc Natl Acad Sci U S A
主要な著者: Eisenberg-Domovich, Yael, Pazy, Yael, Nir, Orit, Raboy, Bilha, Bayer, Edward A., Wilchek, Meir, Livnah, Oded
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 2004
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC395898/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15079055/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0308541101
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