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Rapid, efficient and precise allele replacement in the fission yeast Schizosaccharomyces pombe
Gene targeting provides a powerful tool to modify endogenous loci to contain specific mutations, insertions and deletions. Precise allele replacement, with no other chromosomal changes (e.g., insertion of selectable markers or heterologous promoters), maintains physiologically relevant context. Esta...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3954454/ https://ncbi.nlm.nih.gov/pubmed/24026504 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00294-013-0406-x |
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