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Poly(A) polymerase-based poly(A) length assay
mRNA polyadenylation functions in nuclear export, translation and stability. We describe an efficient protocol designed to assess poly(A) tail length that is based on 3' tailing by yeast poly(A) polymerase and product analysis to single-nucleotide resolution by capillary electrophoresis.
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3951053/ https://ncbi.nlm.nih.gov/pubmed/24590776 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-971-0_2 |
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