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Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells
This chapter describes several methods for measuring the length of the mRNA poly(A) tail and a novel method for measuring mRNA decay. Three methods for measuring the length of a poly(A) tail are presented: the poly(A) length assay, the ligation-mediated poly(A) test (LM-PAT) and the RNase H assay. T...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2734468/ https://ncbi.nlm.nih.gov/pubmed/19111191 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(08)02624-4 |
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