Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning

The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert. A Type IIS endonuclease - a Body Double of the Type IIP enzyme - is used to generate the same protruding palindrom...

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Detalhes bibliográficos
Main Authors: Tóth, Eszter, Huszár, Krisztina, Bencsura, Petra, Kulcsár, Péter István, Vodicska, Barbara, Nyeste, Antal, Welker, Zsombor, Tóth, Szilvia, Welker, Ervin
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3949710/
https://ncbi.nlm.nih.gov/pubmed/24618593
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0090896
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