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Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.

A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiog...

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Bibliografski detalji
Glavni autori: Lane, M J, Dabrowiak, J C, Vournakis, J N
Format: Artigo
Jezik:Inglês
Izdano: 1983
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC394020/
https://ncbi.nlm.nih.gov/pubmed/6304702
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