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Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.
A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiog...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1983
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC394020/ https://ncbi.nlm.nih.gov/pubmed/6304702 |
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