Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.

A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiog...

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Detaylı Bibliyografya
Asıl Yazarlar: Lane, M J, Dabrowiak, J C, Vournakis, J N
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1983
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC394020/
https://ncbi.nlm.nih.gov/pubmed/6304702
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