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Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.

A direct approach to determining the sequence specificities of equilibrium binding drugs by using the DNase protection technique is described. The method utilizes singly end-labeled restriction fragments and partial digestion of the drug fragment complex with DNase I. Microdensitometry of autoradiog...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Lane, M J, Dabrowiak, J C, Vournakis, J N
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394020/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6304702/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.11.3260
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