Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative freque...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1983
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6304690/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.10.3010 |
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