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Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.

A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative freque...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Razzaque, A, Mizusawa, H, Seidman, M M
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393963/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6304690/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.10.3010
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