Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.

A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative freque...

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Bibliographic Details
Main Authors: Razzaque, A, Mizusawa, H, Seidman, M M
Format: Artigo
Language:Inglês
Published: 1983
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC393963/
https://ncbi.nlm.nih.gov/pubmed/6304690
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