Using ubiquitin to follow the metabolic fate of a protein.
We describe a method that can be used to produce equimolar amounts of two or more specific proteins in a cell. In this approach, termed the ubiquitin/protein/reference (UPR) technique, a reference protein and a protein of interest are synthesized as a polyprotein separated by a ubiquitin moiety. Thi...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
1996
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39378/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8643502/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.10.4907 |
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