Screening for in vivo protein-protein interactions.
We describe an in vivo approach for the isolation of proteins interacting with a protein of interest. The protein of interest is "tagged" with a portion of the biotin carboxylase carrier protein (BCCP), encoded on a specially constructed plasmid, so that it becomes biotinylated in vivo. The "query"...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1993
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8430108/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.90.3.933 |
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