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Screening for in vivo protein-protein interactions.

We describe an in vivo approach for the isolation of proteins interacting with a protein of interest. The protein of interest is "tagged" with a portion of the biotin carboxylase carrier protein (BCCP), encoded on a specially constructed plasmid, so that it becomes biotinylated in vivo. Th...

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Bibliografske podrobnosti
Main Authors: Germino, F J, Wang, Z X, Weissman, S M
Format: Artigo
Jezik:Inglês
Izdano: 1993
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC45784/
https://ncbi.nlm.nih.gov/pubmed/8430108
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