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Detection of Transient In Vivo Interactions between Substrate and Transporter during Protein Translocation into the Endoplasmic Reticulum

The split-ubiquitin technique was used to detect transient protein interactions in living cells. N(ub), the N-terminal half of ubiquitin (Ub), was fused to Sec62p, a component of the protein translocation machinery in the endoplasmic reticulum of Saccharomyces cerevisiae. C(ub), the C-terminal half...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Dünnwald, Martin, Varshavsky, Alexander, Johnsson, Nils
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Cell Biology 1999
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC25172/
https://ncbi.nlm.nih.gov/pubmed/9950680
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