Detection of Transient In Vivo Interactions between Substrate and Transporter during Protein Translocation into the Endoplasmic Reticulum
The split-ubiquitin technique was used to detect transient protein interactions in living cells. N(ub), the N-terminal half of ubiquitin (Ub), was fused to Sec62p, a component of the protein translocation machinery in the endoplasmic reticulum of Saccharomyces cerevisiae. C(ub), the C-terminal half...
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| 發表在: | Mol Biol Cell |
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| Principais autores: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Cell Biology
1999
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC25172/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9950680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1091/mbc.10.2.329 |
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