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DNA polymerase preference determines PCR priming efficiency

BACKGROUND: Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of...

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Detalhes bibliográficos
Main Authors: Pan, Wenjing, Byrne-Steele, Miranda, Wang, Chunlin, Lu, Stanley, Clemmons, Scott, Zahorchak, Robert J, Han, Jian
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3937175/
https://ncbi.nlm.nih.gov/pubmed/24479830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-10
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