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DNA polymerase preference determines PCR priming efficiency

BACKGROUND: Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of...

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Hlavní autoři: Pan, Wenjing, Byrne-Steele, Miranda, Wang, Chunlin, Lu, Stanley, Clemmons, Scott, Zahorchak, Robert J, Han, Jian
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3937175/
https://ncbi.nlm.nih.gov/pubmed/24479830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-10
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