DNA polymerase preference determines PCR priming efficiency

BACKGROUND: Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of...

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Autores principales: Pan, Wenjing, Byrne-Steele, Miranda, Wang, Chunlin, Lu, Stanley, Clemmons, Scott, Zahorchak, Robert J, Han, Jian
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3937175/
https://ncbi.nlm.nih.gov/pubmed/24479830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-10
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