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A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase.

A rapid method for efficiently generating site-directed mutations on a clean sequence background is described. This modification of the megaprimer PCR mutagenesis approach can be performed in one tube in less than 4.5 hours, and does not require purification of intermediate products. High fidelity o...

詳細記述

保存先:
書誌詳細
主要な著者: Picard, V, Ersdal-Badju, E, Lu, A, Bock, S C
フォーマット: Artigo
言語:Inglês
出版事項: 1994
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC308213/
https://ncbi.nlm.nih.gov/pubmed/8041621
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