A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase.
A rapid method for efficiently generating site-directed mutations on a clean sequence background is described. This modification of the megaprimer PCR mutagenesis approach can be performed in one tube in less than 4.5 hours, and does not require purification of intermediate products. High fidelity o...
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| Izdano u: | Nucleic Acids Res |
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| Glavni autori: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1994
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| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308213/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8041621/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.13.2587 |
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