A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase.
A rapid method for efficiently generating site-directed mutations on a clean sequence background is described. This modification of the megaprimer PCR mutagenesis approach can be performed in one tube in less than 4.5 hours, and does not require purification of intermediate products. High fidelity o...
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| Опубліковано в:: | Nucleic Acids Res |
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| Автори: | , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
1994
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| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308213/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8041621/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.13.2587 |
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