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Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation
Here we describe a fluctuation-based method to quantify how protein oligomerisation modulates signalling activity of a multifunctional protein. By recording fluorescence lifetime imaging microscopy (FLIM) data of a FRET biosensor in a format that enables concomitant phasor and cross Number and Brigh...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3936235/ https://ncbi.nlm.nih.gov/pubmed/24573109 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04219 |
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