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Design of ultrasensitive probes for human neutrophil elastase through hybrid combinatorial substrate library profiling

The exploration of protease substrate specificity is generally restricted to naturally occurring amino acids, limiting the degree of conformational space that can be surveyed. We substantially enhanced this by incorporating 102 unnatural amino acids to explore the S1–S4 pockets of human neutrophil e...

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Autori principali: Kasperkiewicz, Paulina, Poreba, Marcin, Snipas, Scott J., Parker, Heather, Winterbourn, Christine C., Salvesen, Guy S., Drag, Marcin
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3932852/
https://ncbi.nlm.nih.gov/pubmed/24550277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1318548111
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