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Design of ultrasensitive probes for human neutrophil elastase through hybrid combinatorial substrate library profiling

The exploration of protease substrate specificity is generally restricted to naturally occurring amino acids, limiting the degree of conformational space that can be surveyed. We substantially enhanced this by incorporating 102 unnatural amino acids to explore the S1–S4 pockets of human neutrophil e...

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Detalhes bibliográficos
Main Authors: Kasperkiewicz, Paulina, Poreba, Marcin, Snipas, Scott J., Parker, Heather, Winterbourn, Christine C., Salvesen, Guy S., Drag, Marcin
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3932852/
https://ncbi.nlm.nih.gov/pubmed/24550277
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1318548111
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