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Label-free mass spectrometry exploits dozens of detected peptides to quantify lamins in wildtype and knockdown cells
Label-free quantitation and characterization of proteins by mass spectrometry (MS) is now feasible, especially for moderately expressed structural proteins such as lamins that typically yield dozens of tryptic peptides from tissue cells. Using standard cell culture samples, we describe general algor...
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Main Authors: | , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Landes Bioscience
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3925690/ https://ncbi.nlm.nih.gov/pubmed/24448480 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/nucl.27413 |
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