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Label-free mass spectrometry exploits dozens of detected peptides to quantify lamins in wildtype and knockdown cells

Label-free quantitation and characterization of proteins by mass spectrometry (MS) is now feasible, especially for moderately expressed structural proteins such as lamins that typically yield dozens of tryptic peptides from tissue cells. Using standard cell culture samples, we describe general algor...

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Dettagli Bibliografici
Autori principali: Swift, Joe, Harada, Takamasa, Buxboim, Amnon, Shin, Jae-Won, Tang, Hsin-Yao, Speicher, David W, Discher, Dennis E
Natura: Artigo
Lingua:Inglês
Pubblicazione: Landes Bioscience 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3925690/
https://ncbi.nlm.nih.gov/pubmed/24448480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/nucl.27413
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