Kinetic evidence for an intermediate in the deacetylation of monoacetyl-chymotrypsin.

Mono[14C]acetyl-chymotrypsin was prepared by treating alpha-chymotrypsin with a 10-fold molar excess of p-nitrophenyl[14C]acetate at pH 5, and the acetylated enzyme was isolated free of excess reagents by gel filtration. Deacetylation at pH 6.0 was followed by observing the decrease in acid-precipit...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Chibber, B A, Tomich, J M, Mertz, E T, Viswanatha, T
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 1977
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392319/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/265516/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.2.510
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