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Microtubule Assembly in the Absence of Added Nucleotides

Microtubule assembly is enhanced by the addition of 1 M sucrose or 4 M glycerol to the reassembly mixture. Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions. The tubules assembled in glycerol and sucrose are more stable...

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Detalhes bibliográficos
Main Authors: Shelanski, Michael L., Gaskin, Felicia, Cantor, Charles R.
Formato: Artigo
Idioma:Inglês
Publicado em: 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC433354/
https://ncbi.nlm.nih.gov/pubmed/4514990
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