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Microtubule Assembly in the Absence of Added Nucleotides
Microtubule assembly is enhanced by the addition of 1 M sucrose or 4 M glycerol to the reassembly mixture. Tubulin can be purified from guinea pig brain readily and in good yield by two cycles of assembly in glycerol-containing solutions. The tubules assembled in glycerol and sucrose are more stable...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1973
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC433354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4514990/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.3.765 |
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