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In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol.

Microtubule protein from porcine cerebrum was fractionate into pure tubulin and microtubule-associated proteins by chromatography on phosphocellulose. In agreement with previous studies, pure tubulin does not form microtubules to a significant extent at 37 degrees in normal assembly buffers, which a...

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Detalhes bibliográficos
Main Authors: Herzog, W, Weber, K
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC431031/
https://ncbi.nlm.nih.gov/pubmed/266708
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