In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol.
Microtubule protein from porcine cerebrum was fractionate into pure tubulin and microtubule-associated proteins by chromatography on phosphocellulose. In agreement with previous studies, pure tubulin does not form microtubules to a significant extent at 37 degrees in normal assembly buffers, which a...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1977
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC431031/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/266708/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.5.1860 |
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