Kinetic evidence for an intermediate in the deacetylation of monoacetyl-chymotrypsin.
Mono[14C]acetyl-chymotrypsin was prepared by treating alpha-chymotrypsin with a 10-fold molar excess of p-nitrophenyl[14C]acetate at pH 5, and the acetylated enzyme was isolated free of excess reagents by gel filtration. Deacetylation at pH 6.0 was followed by observing the decrease in acid-precipit...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1977
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392319/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/265516/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.2.510 |
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