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Kinetic evidence for an intermediate in the deacetylation of monoacetyl-chymotrypsin.

Mono[14C]acetyl-chymotrypsin was prepared by treating alpha-chymotrypsin with a 10-fold molar excess of p-nitrophenyl[14C]acetate at pH 5, and the acetylated enzyme was isolated free of excess reagents by gel filtration. Deacetylation at pH 6.0 was followed by observing the decrease in acid-precipit...

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Bibliografiske detaljer
Main Authors: Chibber, B A, Tomich, J M, Mertz, E T, Viswanatha, T
Format: Artigo
Sprog:Inglês
Udgivet: 1977
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC392319/
https://ncbi.nlm.nih.gov/pubmed/265516
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