Stoichiometry of GTP hydrolysis and tubulin polymerization.
Microtubule formation from lamb brain tubulin isolated by affinity chromatography and freed of exchangeable nucleotide requires GTP for maximal rate and extent of polymerization. The nucleotide analogs guanylylmethylenediphosphate and guanylylimidodiphosphate fail to replace GTP; in addition, neithe...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1977
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC392309/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/191810/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.2.462 |
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