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Side chain dynamics of carboxyl and carbonyl groups in the catalytic function of Escherichia coli ribonuclease H

Many proteins use Asx and Glx (x = n, p, or u) side chains as key functional groups in enzymatic catalysis and molecular recognition. In this study, NMR spin relaxation experiments and molecular dynamics (MD) simulations are used to measure the dynamics of the side chain amide and carboxyl groups, (...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Stafford, Kate A., Ferrage, Fabien, Cho, Jae-Hyun, Palmer, Arthur G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3918730/
https://ncbi.nlm.nih.gov/pubmed/24219366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja409479y
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