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Amino acid substitution in the active site of DNA polymerase β explains the energy barrier of the nucleotidyl transfer reaction

DNA polymerase β (pol β) is a bifunctional enzyme widely studied for its roles in base excision DNA repair where one key function is gap-filling DNA synthesis. In spite of significant progress in recent years, the atomic level mechanism of the DNA synthesis reaction has remained poorly understood. B...

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Hlavní autoři: Batra, Vinod K., Perera, Lalith, Lin, Ping, Shock, David D., Beard, William A., Pedersen, Lars C., Pedersen, Lee G., Wilson, Samuel H.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3918438/
https://ncbi.nlm.nih.gov/pubmed/23647366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja403842j
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