Structures of DNA Polymerase β with Active Site Mismatches Suggest a Transient Abasic Site Intermediate During Misincorporation

We report the crystallographic structures of DNA polymerase β with dG—dAMPCPP and dC— dAMPCPP mismatches in the active site. These pre-mutagenic structures were obtained with a non-hydrolysable incoming nucleotide analog, dAMPCPP, and Mn(+2). Substituting Mn(2+) for Mg(2+) significantly decreases th...

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Autores principales: Batra, Vinod K., Beard, William A., Shock, David D., Pedersen, Lars C., Wilson, Samuel H.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2008
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2399898/
https://ncbi.nlm.nih.gov/pubmed/18471977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2008.02.025
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