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The Neutron Structure of Urate Oxidase Resolves a Long-Standing Mechanistic Conundrum and Reveals Unexpected Changes in Protonation

Urate oxidase transforms uric acid to 5-hydroxyisourate without the help of cofactors, but the catalytic mechanism has remained enigmatic, as the protonation state of the substrate could not be reliably deduced. We have determined the neutron structure of urate oxidase, providing unique information...

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Bibliografiske detaljer
Main Authors: Oksanen, Esko, Blakeley, Matthew P., El-Hajji, Mohamed, Ryde, Ulf, Budayova-Spano, Monika
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3900588/
https://ncbi.nlm.nih.gov/pubmed/24466188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0086651
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