Joint neutron/X-ray crystal structure of a mechanistically relevant complex of perdeuterated urate oxidase and simulations provide insight into the hydration step of catalysis

Cofactor-independent urate oxidase (UOX) is an ∼137 kDa tetrameric enzyme essential for uric acid (UA) catabolism in many organisms. UA is first oxidized by O(2) to de­hydro­isourate (DHU) via a peroxo intermediate. DHU then undergoes hydration to 5-hy­droxy­isourate (5HIU). At different stages of t...

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Bibliografske podrobnosti
izdano v:IUCrJ
Main Authors: McGregor, Lindsay, Földes, Tamás, Bui, Soi, Moulin, Martine, Coquelle, Nicolas, Blakeley, Matthew P., Rosta, Edina, Steiner, Roberto A.
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2021
Teme:
Research Papers
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7792999/
https://ncbi.nlm.nih.gov/pubmed/33520242
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2052252520013615
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