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In Vivo Evolution of a Catalytic RNA Couples Trans-Splicing to Translation
How does a non-coding RNA evolve in cells? To address this question experimentally we evolved a trans-splicing variant of the group I intron ribozyme from Tetrahymena over 21 cycles of evolution in E.coli cells. Sequence variation was introduced during the evolution by mutagenic and recombinative PC...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3900562/ https://ncbi.nlm.nih.gov/pubmed/24466112 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0086473 |
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