Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate

Mitochondrial flux is currently accessible at low resolution. Here we introduce a genetically-encoded FRET sensor for pyruvate, and methods for quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resol...

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Detaylı Bibliyografya
Asıl Yazarlar: San Martín, Alejandro, Ceballo, Sebastián, Baeza-Lehnert, Felipe, Lerchundi, Rodrigo, Valdebenito, Rocío, Contreras-Baeza, Yasna, Alegría, Karin, Barros, L. Felipe
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2014
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3897509/
https://ncbi.nlm.nih.gov/pubmed/24465702
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0085780
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