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Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate
Mitochondrial flux is currently accessible at low resolution. Here we introduce a genetically-encoded FRET sensor for pyruvate, and methods for quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resol...
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| Autori principali: | , , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Public Library of Science
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3897509/ https://ncbi.nlm.nih.gov/pubmed/24465702 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0085780 |
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