Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate

Mitochondrial flux is currently accessible at low resolution. Here we introduce a genetically-encoded FRET sensor for pyruvate, and methods for quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resol...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: San Martín, Alejandro, Ceballo, Sebastián, Baeza-Lehnert, Felipe, Lerchundi, Rodrigo, Valdebenito, Rocío, Contreras-Baeza, Yasna, Alegría, Karin, Barros, L. Felipe
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Public Library of Science 2014
Pynciau:
Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3897509/
https://ncbi.nlm.nih.gov/pubmed/24465702
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0085780
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